The Polygon interview explores how Coyote vs. ACME's filmmakers deliberately revived the original, more mischievous version of Tweety Bird from his earliest Looney Tunes shorts, casting him against type as a villainous enforcer for the ACME Corporation. This creative choice, along with a similarly faithful take on a wilder, Bob Clampett-style Daffy Duck, underpins the film's most talked-about scene, in which John Cena's character confronts Tweety in the desert and mimics his distinctive speech pattern.

Director Dave Green and screenwriter Samy Burch explain that Tweety was conceived as a "heavy" from the earliest pitch, drawing on his sadistic streak from his debut cartoon rather than the sweeter, more recent portrayals audiences expect. The now-famous desert standoff scene, shot in Albuquerque on a backdrop familiar from Breaking Bad and Better Call Saul, was actually a late rewrite forced by Will Forte falling ill during filming; Green praises Cena for playing the absurd Tweety-speak dialogue completely deadpan.